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KMID : 1134820220510101109
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 10 p.1109 ~ p.1117
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Contents of Isoflavone in a Prime Core Set of Korean Soybean Germpla
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Jeon Jin-Soo
Jang Young-Hyeon
Choung Myoung-Gun
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Abstract
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We analyzed 12 kinds of isoflavones contained in a prime core set of Korean soybean germplasms using high-performance liquid chromatography with diode-array detection (HPLC/DAD). Malonylgenistin (MGE) accounted for the highest content at 129.80∼3,437.88 μg/g, malonyldaidzin (MDA) was 97.78∼3,231.85 μg/g, and the total isoflavone content was 255.29∼6,707.23 μg/g. Principal component analysis was performed to confirm that CMJ17105, CMJ17407, and CMJ17359 had high isoflavone content among the core set of germplasms. As a result of Pearson’s correlation analysis, MDA (r=0.919***), acetyldaidzin (ADA; r=0.923***), and MGE (r=0.943***) showed a high correlation of significance with total isoflavone. Using a heatmap including cluster analysis, a core set of soybean germplasms was divided into 5 groups according to the isoflavone content and composition. This study provides information on a core set of germplasms with high isoflavone content. This information can be useful for breeding high-quality soybean cultivars and developing soy processed foods.
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KEYWORD
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soybean, germplasm, core set, isoflavone, HPLC
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